HPTLC AMD OPLC Chromatography Submerged cultures Carbohydrates analysis Complex matrix Introductionīiotechnology industries are based on exploiting the metabolic activities of microbes, plants and animal cells to produce a wide variety of different compounds, which are used by other industries such as chemical, food, pharmaceutical and health care. Results could be easily extended to other fermentation processes. was used, a saccharolytic bacterium with probiotic activities in the human gut, able to anaerobically ferment complex sugar mixtures. As a model of submerged fermentations, a strain of Bifidobacterium spp. The methods set up were then applied for quantitative determination of sugars. Four different carbohydrates complex mixtures were analyzed using HPTLC techniques, as to optimize the quality of the separation among components. HPTLC methods development using instrumental techniques as OPLC (Over Pressure Liquid Chromatography) and AMD (Automated Multiple Development) has been also described, as to simultaneously monitor several samples in the same elution, with significant time and solvent savings. ![]() The documentation of plates was performed using TLC visualization device and the images of plates were processed using a digital processor. The proposed method involved the chromatographic separations of xilo-, galacto-, fructo-oligosaccharides mixtures at different molecular weights, tri- and disaccharides (raffinose, sucrose, lactose), and the corresponding monosaccharides (xylose, fructose, galactose) on HPTLC plates, using different eluent mixtures and elution conditions. HPTLC is a widely used, fast and accurate method of separating complex mixtures. For this reason, the objective of this study is to develop and validate a simple and relatively economical analytical method for detecting complex sugar mixtures in fermentation broth based on High-Performance Thin-Layer Chromatography (HPTLC). A method for detection and quantification of sugar levels are very advantageous because they can be considered as key indicators in determining the yields and the productivity of the process. Visit for more related articles at Journal of Analytical & Bioanalytical TechniquesĬarbohydrates are the principal sources of nutrient and energy in large-scale submerged fermentation processes. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received date: JAccepted date: JPublished date: June 23, 2015Ĭitation: Bernardi T, Pedrini P, Marchetti MG, Tamburini E (2015) Separation and Quantitative Determination of Carbohydrates in Microbial Submerged Cultures Using Different Planar Chromatography Techniques (HPTLC, AMD, OPLC). Tatiana Bernardi 1, Paola Pedrini 2, Maria Gabriella Marchetti 2 and Elena Tamburini 2 *ġ Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, ItalyĢ Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy *Corresponding Author: Elena Tamburiniĭepartment of Life Sciences and BBiotechnology Submerged Cultures Using Different Planar Chromatography Techniques GIS file, Page Layout file, Plugin file, Settings file, Spreadsheet, System file, Text, Web Format File & Misc file(doesn't fit in any other category) such as '.torrent'.Separation and Quantitative Determination of Carbohydrates in Microbial ![]() Evaluates and classifies file type based on it's extension as Īudio,Video,Raster Image, Vector Image, 3D Image, Backup file, CAD file, RAW Camera file, Compressed, Data file, Developer file, Disk Image, eBook, Encoded, Executable, Font, Game file,
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